CORRECTED Ligation : Sometimes unnecessarily produced. NOT FOR USE IN DIAGNOSTIC PROCEDURES (EXCEPT AS SPECIFICALLY NOTED). Serial Cloner, DNA, plasmid, cloning, PCR, NCBI, Genbank, Vector NTI, MacVector, restriction. Our mission is to develop high-quality innovative tools and services to accelerate discovery.įOR RESEARCH USE ONLY. As a member of the Takara Bio Group, Takara Bio USA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology. More recent cloning methodologies utilize other properties of DNA polymerases, such as exonuclease activity, along with homologous regions of DNA that join seamlessly together. Generally, this strategy implies that each coding sequence is cloned in a ‘donor’ plasmid flanked by unique restriction sites. provides kits, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function. Until recently, classical restriction enzyme-based cloning was the method of choice for assembly of poly-cistronic vectors for heterologous expression of recombinant protein complexes 44, 49, 50, 51.
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